TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants

@article{Wendt2013TALEN,
  title={TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants},
  author={Toni Wendt and Preben Bach Holm and Colby Starker and Michelle L. Christian and Daniel F. Voytas and Henrik Brinch-Pedersen and Inger B Holme},
  journal={Plant Molecular Biology},
  year={2013},
  volume={83},
  pages={279-285}
}
Transcription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of organisms. The primary advantage of TALENs over other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their ease of assembly, reliability of function, and their broad targeting range. Here we report the assembly of several TALENs for a specific genomic locus in barley. The cleavage activity of individual TALENs was first tested in vivo using a yeast-based… CONTINUE READING
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