T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication

@article{Liu1979T4DT,
  title={T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication},
  author={L. Liu and Chung-Cheng Liu and B. Alberts},
  journal={Nature},
  year={1979},
  volume={281},
  pages={456-461}
}
A novel ATP-dependent DNA topoisomerase which makes reversible double-strand breaks in the DNA double helix has been purified to near homogeneity from T4 bacteriophage-infected Escherichia coli cells. Genetic data suggest that this activity is essential for initiating T4 DNA replication forks in vivo. 
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