T3-sequencing: targeted characterization of the N- and C-termini of undigested proteins by mass spectrometry.

@article{Suckau2003T3sequencingTC,
  title={T3-sequencing: targeted characterization of the N- and C-termini of undigested proteins by mass spectrometry.},
  author={Detlev Suckau and Anja Resemann},
  journal={Analytical chemistry},
  year={2003},
  volume={75 21},
  pages={5817-24}
}
A novel extension of the "top-down" approach is introduced for the selective characterization of protein termini that does not involve proteolytic digestion steps. N- and C-terminal peptides were generated from intact proteins in the mass spectrometer and further analyzed by MS/MS-an approach referred to as T(3)-sequencing. N-terminal and C-terminal fragment ion series were obtained by the pseudo-MS/MS technique in-source decay (ISD) on a matrix-assisted laser desorption/ionization time-of… CONTINUE READING

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