T-dependence of human B lymphocyte proliferative response to mitogens.

  title={T-dependence of human B lymphocyte proliferative response to mitogens.},
  author={Jean Brochier and Christiane Samarut and J P Gueho and Jean Pierre Revillard},
  journal={International archives of allergy and applied immunology},
  volume={51 1},
Human peripheral blood and tonsil lymphocytes were fractionated on anti-Ig-coated Sephadex columns or by centrifugation after rosetting with native sheep erythrocytes. Both methods allowed the recovery of B and T-enriched populations the purity of which was checked by fluorescein-labelled anti-Ig serum, E and EAC rosette formation, and heterologous antisera specific for B or T lymphocytes. The proliferative response of T cells to PHA, Con A, PWM, and ALS was not found different from that of… CONTINUE READING