T cells migrate to tumour sites after extracorporeal interleukin 2 stimulation and reinfusion in a patient with metastatic melanoma.

Abstract

Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3+). After radiolabelling with indium-111, the cells were reinfused. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25+ T lymphocytes (CD2+, CD3+), but no natural killer cells (CD16+, CD56+) infiltrating the metastases. LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient.

Statistics

05001000'98'00'02'04'06'08'10'12'14'16
Citations per Year

98 Citations

Semantic Scholar estimates that this publication has 98 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Dummer1993TCM, title={T cells migrate to tumour sites after extracorporeal interleukin 2 stimulation and reinfusion in a patient with metastatic melanoma.}, author={Reinhard Dummer and J{\"{u}rgen C Becker and Chr Eilles and E. L. Sch{\"a}fer and Wilhelm B{\"{o}rner and Guenter Burg}, journal={The British journal of dermatology}, year={1993}, volume={128 4}, pages={399-403} }