T cell receptor V beta gene usage in allograft-derived cell lines analyzed by a polymerase chain reaction technique.

@article{Hall1992TCR,
  title={T cell receptor V beta gene usage in allograft-derived cell lines analyzed by a polymerase chain reaction technique.},
  author={Bruce Lee Hall and O J Finn},
  journal={Transplantation},
  year={1992},
  volume={53 5},
  pages={1088-99}
}
T cell receptor V beta gene usage in renal allograft-derived T cell lines was investigated using a semiquantitative, polymerase chain reaction (PCR)-based technique. A panel of V beta-specific primers was carefully designed to have uniform hybridization characteristics, enhancing comparability, and high specificity based on 3' mismatches between primers and nontarget V regions. PCR data were in concordance with previous phenotypic, functional, and Southern blot analyses, but the PCR technique… CONTINUE READING