T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.

@article{Chilton1980TDNAFA,
  title={T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.},
  author={Marcia Chilton and Randall K. Saiki and N V Yadav and Milton Paul Gordon and Françis Qu{\'e}tier},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 7},
  pages={4060-4}
}
The crown gall teratoma tumor line BT37, incited by Agrobacterium tumefaciens strain T37, has been found to contain part of the tumor-inducing plasmid, pTi T37, of the inciting strain. This foreign DNA segment, called T-DNA, is maintained at several copies per diploid tumor cell. We have examined subcellular DNA fractions from this tumor line in an effort to determine whether T-DNA is in chloroplasts, mitochondria, or nuclei. Tumor cell chloroplast DNA exhibited EcoRI and Bst I endonuclease… CONTINUE READING

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