T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange

@article{Fan2011TDNADR,
  title={T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange},
  author={Jing Fan and Xin Liu and Shi-xiao Xu and Qiang Xu and Wen-wu Guo},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2011},
  volume={107},
  pages={225-232}
}
In transgenic plants, multiple T-DNA copies and cytosine methylation usually correlate with a loss of transgene expression while methylation of promoter is associated with transgene silencing. Here, six independent GFP transgenic ‘Bingtang’ sweet orange (Citrus sinensis (L.) Osb.) plants were analyzed with copy number, T-DNA repeat, cytosine methylation and transgene expression. The fluorescence of transgenic plants was normal and bright except that the transgenic B4 plant showed mottled… CONTINUE READING