T-Cell receptor Vbeta repertoire CDR3 length diversity differs within CD45RA and CD45RO T-cell subsets in healthy and human immunodeficiency virus-infected children.

@article{Kou2000TCellRV,
  title={T-Cell receptor Vbeta repertoire CDR3 length diversity differs within CD45RA and CD45RO T-cell subsets in healthy and human immunodeficiency virus-infected children.},
  author={Zhong Chen Kou and Joshua S Puhr and Marie Rojas and W T Mccormack and Maureen Michels Goodenow and John William Sleasman},
  journal={Clinical and diagnostic laboratory immunology},
  year={2000},
  volume={7 6},
  pages={953-9}
}
The T-cell receptor (TCR) CDR3 length heterogeneity is formed during recombination of individual Vbeta gene families. We hypothesized that CDR3 length diversity could be used to assess the fundamental differences within the TCR repertoire of CD45RA and CD45RO T-cell subpopulations. By using PCR-based spectratyping, nested primers for all 24 human Vbeta families were developed to amplify CDR3 lengths in immunomagnetically selected CD45RA and CD45RO subsets within both CD4(+) and CD8(+) T-cell… CONTINUE READING