Systems-wide Analysis of a Phosphatase Knock-down by Quantitative Proteomics and Phosphoproteomics

  title={Systems-wide Analysis of a Phosphatase Knock-down by Quantitative Proteomics and Phosphoproteomics},
  author={Maximiliane Hilger and Tiziana Bonaldi and Florian Gnad and Matthias Mann},
  booktitle={Molecular & cellular proteomics : MCP},
Signal transduction in metazoans regulates almost all aspects of biological function, and aberrant signaling is involved in many diseases. Perturbations in phosphorylation-based signaling networks are typically studied in a hypothesis-driven approach, using phospho-specific antibodies. Here we apply quantitative, high-resolution mass spectrometry to determine the systems response to the depletion of one signaling component. Drosophila cells were metabolically labeled using stable isotope… CONTINUE READING


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