Systems-level understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: mechanism and application.

@article{Guan2013SystemslevelUO,
  title={Systems-level understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: mechanism and application.},
  author={Ningzi Guan and Long Liu and Hyun-dong Shin and Rachel Chen and Juan Zhang and Jianghua Li and Guocheng Du and Zhongping Shi and Jian Chen},
  journal={Journal of biotechnology},
  year={2013},
  volume={167 1},
  pages={
          56-63
        }
}
In previous work, three evolved Propionibacterium acidipropionici mutants with higher tolerant capacity of propionic acid (PA) were obtained by genome shuffling. Here, we attempted to unravel the acid-tolerant mechanism of P. acidipropionici by comparing the physiological changes between P. acidipropionici and three mutants. The parameters used for comparison included intracellular pH (pHi), NAD⁺/NADH ratio, H⁺-ATPase activity, and the intracellular amino acids concentrations. It was indicated… Expand
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