Systemic movement of a tobamovirus requires host cell pectin methylesterase.

  title={Systemic movement of a tobamovirus requires host cell pectin methylesterase.},
  author={Min‐Huei Chen and Vitaly Citovsky},
  journal={The Plant journal : for cell and molecular biology},
  volume={35 3},
The analysis of virus distribution in the PME antisense plants suggested that TMV systemic movement may be a polar process in which the virions enter and exit the vascular system by two different mechanisms, and it is the viral exit out of theascular system that involves PME.

Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis.

In tobacco plants constitutively expressing a PMEI from Actinidia chinensis (AcPMEI), systemic movement of Tobacco mosaic virus (TMV) is limited and viral symptoms are reduced and PMEIs are able to limit tobamovirus movement and to reduce plant susceptibility to the virus.

The Cowpea mosaic virus movement protein

The research described in this thesis focused on the functioning of the CPMV MP with special emphasis on its interactions with virion proteins and host proteins.

Pectin Methylesterase Enhances Tomato Bushy Stunt Virus P19 RNA Silencing Suppressor Effects

This chapter shows that PME can prevent nuclear transport of reporter proteins, GFP and GFP-tagged TMV movement protein (MP) and MS2 phage coat protein fused with a nuclear localization signal (NLS).

How do pectin methylesterases and their inhibitors affect the spreading of tobamovirus?

How overexpression of PMEI may reduce tobamovirus spreading is discussed, which reduces plant susceptibility to these viruses.

NTH201, a novel class II KNOTTED1-like protein, facilitates the cell-to-cell movement of Tobacco mosaic virus in tobacco.

Results suggested that NTH201 might indirectly facilitate MP accumulation and VRC formation in TMV-infected cells, leading to rapid viral cell-to-cell movement in plants at an early infection stage.

Viral and Cellular Factors Involved in Phloem Transport of Plant Viruses

While most viruses seem to move systemically as virus particles, some viruses are transported in SE as viral ribonucleoprotein complexes (RNP).

Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana

The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.

Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans

This is the first report on identification and characterization of a PME gene within the phylum Nematoda, pectin methylesterase PME (EC 3.1.11), in the root lesion nematode Pratylenchus penetrans.

The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

It is hypothesized that plant wounding and/or pathogen attack lead to PME activation and increased methanol emission, followed by increased NbAELP expression, which results in reversion of PME mRNA level and methanl emission to levels found in the intact plant.

Physiological effects of constitutive expression of Oilseed Rape Mosaic Tobamovirus (ORMV) movement protein in Arabidopsis thaliana

The transgenic Arabidopsis plants were susceptible to ORMV infection, although showing milder overall symptoms than non-transgenic controls, and highlight the relevance of the specific host-virus system, in the physiological outcome of the molecular interactions established by MPs.

Movement and subcellular localization of a tobamovirus in Arabidopsis.

The ability to move both locally and systemically in Arabidopsis, causing mild and fast-developing symptoms but allowing survival and fertility of the infected plants, distinguish TVCV infection of Arabidops as a model system to study virus-plant interaction.

The systemic movement of a tobamovirus is inhibited by a cadmium-ion-induced glycine-rich protein

A tobacco glycine-rich protein, cdiGRP, specifically induced by low concentrations of cadmium and expressed in the cell walls of plant vascular tissues may function to control plant viral systemic movement.

Plasmodesmata in Relation to Viral Movement within Leaf Tissues

Although the ultra­ structural approach to the study of viral infection provided essential knowledge on the cytopathological effects of viruses and their cellular distribution, within the plant, it did not provide information on the actual mechanism(s) involved in viral movement.

Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell‐to‐cell movement

A cell wall‐associated protein that specifically binds the viral MP was purified from tobacco leaf cell walls and identified as pectin methylesterase (PME), and deletion of the PME‐binding region resulted in inactivation of TMV cell‐to‐cell movement.

The spread of Tobacco mosaic virus infection: insights into the cellular mechanism of RNA transport

    M. Heinlein
    Biology, Environmental Science
  • 2002
This review summarizes current knowledge of Tobacco mosaic virus infection with emphasis on the mechanism by which this virus targets its RNA genome from sites of replication to plasmodesmata to achieve intercellular spread.

A novel function for a ubiquitous plant enzyme pectin methylesterase : the host-cell receptor for the tobacco mosaic virus movement protein

The present work indicates that a ubiquitous cell wall-associated plant enzyme pectin methylesterase of Nicotiana tabacum L. specifically binds to the movement protein encoded by tobacco mosaic virus, and shows that pect in methylestersterase is an RNA binding protein.

Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants.

The structure and function of plasmodesmata, particularly those aspects related to virus movement, are examined as a function of leaf development in young leaves of 30-kD TMV MP transgenic and vector control plants.

Non-toxic concentrations of cadmium inhibit systemic movement of turnip vein clearing virus by a salicylic acid-independent mechanism.

It was demonstrated that cadmium treatment did not affect TVCV transport from the inoculated non-vascular tissue into the plant vasculature but blocked viral exit into uninoculatedNon-Vascular tissues, suggesting that TVCV virions may enter and exit the host plant vascular system by two different mechanisms.

Vascular invasion routes and systemic accumulation patterns of tobacco mosaic virus in Nicotiana benthamiana.

This work shows the functional equivalence of vein classes in source leaves for entry of Tobacco mosaic virus, and the lack of equivalence in sink leaves for exit of TMV, indicating that the specialization of major veins for transport rather than loading of photoassimilates in source tissue does not preclude virus entry.

Characterization and Functional Expression of a Ubiquitously Expressed Tomato Pectin Methylesterase

The results suggest that there exists developmentally regulated silencing of pmeu1 by a heterologous PME antisense gene, the first evidence, to the authors' knowledge, of the functional characterization of a PME gene and the extensive modification of the encoded polypeptide.