Systemic morphine suppresses noxious stimulus-evoked Fos protein-like immunoreactivity in the rat spinal cord

  title={Systemic morphine suppresses noxious stimulus-evoked Fos protein-like immunoreactivity in the rat spinal cord},
  author={RW Presley and Daniel Men{\'e}trey and J. D. Levine and AI Basbaum},
  booktitle={Journal of Neuroscience},
Previous experiments have shown that noxious stimulation increases expression of the c-fos proto-oncogene in subpopulations of spinal cord neurons. c-fos expression was assessed by immunostaining for Fos, the nuclear phosphoprotein product of the c-fos gene. In this study, we examined the effect of systemic morphine on Fos-like immunoreactivity (FLI) evoked in the formalin test, a widely used model of persistent pain. Awake rats received a subcutaneous 150 microliters injection of 5% formalin… 

Differential effects of morphine on noxious stimulus-evoked fos-like immunoreactivity in subpopulations of spinoparabrachial neurons

Two distinct populations of spinoparabrachial neurons can be recognized on the basis of their expression of the c-fos gene in response to noxious stimulation, suggesting that injury can produce significant molecular changes in neurons even though the neuronal activity and pain associated with the injury is blocked by morphine.

Morphine or U‐50,488 suppresses fos protein‐like immunoreactivity in the spinal cord and nucleus tractus solitarii evoked by a noxious visceral stimulus in the rat

It is suggested that morphine and U‐50,488 have comparable effects on the transmission of visceral nociceptive messages by spinal neurons, but differentially affect the autonomic response to noxious visceral stimuli.



Induction of c-fos-like protein in spinal cord neurons following sensory stimulation

Physiological stimulation of rat primary sensory neurons causes the expression of c-fos-protein-like immunoreactivity in nuclei of postsynaptic neurons of the dorsal horn of the spinal cord, suggesting that synaptic transmission may induce rapid changes in gene expression in certain post Synaptic neurons.

Multiple opioid peptides and the modulation of pain: Immunohistochemical analysis of dynorphin and enkephalin in the trigeminal nucleus caudalis and spinal cord of the cat

Using immunocytochemistry, it is suggested that the dense enkephalin terminal immunoreactivity in the inner part of the substantia gelatinosa derives from cells in lamina III, and there are significant differences in the anatomical relationship of the two opioid peptides with the organization of parasympatheic autonomic preganglionic neurons.

[Peripheral and spinal mechanisms of nociception].

The involvement of contralateral ascending pathways in the transmission of nociceptive messages toward supraspinal structures is well established while the role of ipsilateral ascending systems (spino-cervical and dorsal columns post-synaptic fibers) is still questioned.

Dissociation of supraspinal and spinal actions of morphine: A quantitative evaluation

Mapping patterns of c-fos expression in the central nervous system after seizure.

A dramatic and specific induction of c-fos was observed in identifiable neuronal populations in vivo after administration of the convulsant Metrazole. This effect was time- and dose-dependent and was