Systematic targeted mutagenesis of Brucella melitensis 16M reveals a major role for GntR regulators in the control of virulence.

@article{Haine2005SystematicTM,
  title={Systematic targeted mutagenesis of Brucella melitensis 16M reveals a major role for GntR regulators in the control of virulence.},
  author={Val{\'e}rie Haine and Audrey Sinon and Fr{\'e}d{\'e}ric Van Steen and St{\'e}phanie Rousseau and Marie Dozot and Pascal Lestrate and Christophe Lambert and Jacques Letesson and Xavier De Bolle},
  journal={Infection and immunity},
  year={2005},
  volume={73 9},
  pages={
          5578-86
        }
}
In order to identify transcriptional regulators involved in virulence gene control in Brucella melitensis, we generated a collection of 88 mutants in the AraC, ArsR, Crp, DeoR, GntR, IclR, LysR, MerR, RpiR, and TetR families of regulators. This collection was named LiMuR (library of mutants for regulators). We developed a method to test several mutants simultaneously in one animal in order to identify those unable to survive. This method, called the plasmid-tagged mutagenesis method, was used… CONTINUE READING
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