Systematic screening of an arrayed cDNA library by PCR.

  title={Systematic screening of an arrayed cDNA library by PCR.},
  author={David J. Munroe and Ralf W Loebbert and E Bric and T Whitton and Dirk Prawitt and Dung Vu and Alan Buckler and Andreas Winterpacht and Bernhard Zabel and David Housman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={92 6},
We have developed a PCR-based method for rapid and effective screening of arrayed cDNA libraries. This strategy directly addresses the limitations of conventional hybridization-based schemes and provides a more rapid, cost-effective, and sensitive method compatible with large-scale and routine cDNA clone recovery. To prepare arrayed libraries, 1-2 x 10(6) cDNA clones were propagated as individual plaques on solid medium in 24-well culture dishes at approximately 250 plaque-forming units per… CONTINUE READING


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