Systematic screening of a Drosophila ORF library in vivo uncovers Wnt/Wg pathway components.

@article{Schertel2013SystematicSO,
  title={Systematic screening of a Drosophila ORF library in vivo uncovers Wnt/Wg pathway components.},
  author={Claus Schertel and Dashun Huang and Mikael Bj{\"o}rklund and Johannes Bischof and Dingzi Yin and Rongxia Li and Yi Wu and Rong Zeng and Jiarui Wu and Jussi Taipale and Haiyun Song and Konrad Basler},
  journal={Developmental cell},
  year={2013},
  volume={25 2},
  pages={207-19}
}
We created a site-directed UAS-ORF library of 655 growth-regulating genes in Drosophila. This library represents a large collection of genes regulating cell cycle, cell size, and proliferation and will be a valuable resource for studying growth regulation in vivo. By using misexpression of genes, we prevent problems arising from genetic redundancy and can uncover novel gene functions. To validate the usefulness of this library, we screened for Wingless (Wg) pathway components. We used a… CONTINUE READING
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