Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.

Abstract

Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that favor HDR over NHEJ… (More)
DOI: 10.1038/srep23549

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