Systematic investigation of hierarchical phosphorylation by protein kinase CK2.

@article{StDenis2015SystematicIO,
  title={Systematic investigation of hierarchical phosphorylation by protein kinase CK2.},
  author={Nicole A St-Denis and Michelle Gabriel and Jacob P. Turowec and Gregory B. Gloor and Shawn S. C. Li and Anne-Claude Gingras and David W. Litchfield},
  journal={Journal of proteomics},
  year={2015},
  volume={118},
  pages={49-62}
}
UNLABELLED Although multiple phosphorylation sites are often clustered in substrates, the mechanism of phosphorylation within clusters has not been systematically investigated. Intriguingly, in addition to acidic residues, protein kinase CK2 can use phosphoserine residues as consensus determinants suggesting that CK2 may act in concert with other kinases. We used a peptide array approach to outline optimal consensus sequences for hierarchical phosphorylation by CK2, both in the context of… CONTINUE READING