Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria.

@article{Gmez2010SystematicIO,
  title={Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria.},
  author={Diana Isabel G{\'o}mez and Susan P. Fisher-Hoch and Andrea S. Bordt and Teresa N Quitugua and Jaime G{\'o}mez Robledo and Nataly Alvarez and Nidia E Correa and Joseph B Mccormick and Blanca I Restrepo},
  journal={Diagnostic microbiology and infectious disease},
  year={2010},
  volume={67 1},
  pages={37-46}
}
Detection of multidrug-resistant tuberculosis (MDR-TB), a frequent cause of treatment failure, takes 2 or more weeks to identify by culture. Rifampicin (RIF) resistance is a hallmark of MDR-TB, and detection of mutations in the rpoB gene of Mycobacterium tuberculosis using molecular beacon probes with real-time quantitative polymerase chain reaction (qPCR) is a novel approach that takes </=2 days. However, qPCR identification of resistant isolates, particularly for isolates with mixed RIF… CONTINUE READING