Systematic identification of mitotic phosphoproteins

  title={Systematic identification of mitotic phosphoproteins},
  author={P. Todd Stukenberg and Kevin D. Lustig and Thomas J. McGarry and Randall W. King and Jian Kuang and Marc W Kirschner},
  journal={Current Biology},
BACKGROUND Cyclin-dependent kinases (CDKs) are thought to initiate and coordinate cell division processes by sequentially phosphorylating key targets; in most cases these substrates remain unidentified. RESULTS Using a screen that scores for phosphorylation of proteins, which were translated from pools of cDNA plasmids in vitro, by either phosphoepitope antibody recognition or electrophoretic mobility shifts, we have identified 20 mitotically phosphorylated proteins from Xenopus embryos, 15… CONTINUE READING
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A novel expression cloning strategy for the identification of genes involved in cell cycle control , apoptosis and early

  • KD Lustig, PT Stukenberg, T McGarry, RW King, V Cryns, P Mead
  • development Methods Enzymol
  • 1997

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