Systematic functional analysis of the Caenorhabditis elegans genome using RNAi

@article{Kamath2003SystematicFA,
  title={Systematic functional analysis of the Caenorhabditis elegans genome using RNAi},
  author={R. Kamath and A. Fraser and Y. Dong and G. Poulin and R. Durbin and M. Gotta and A. Kanapin and N. Bot and S. Moreno and M. Sohrmann and David P. Welchman and P. Zipperlen and J. Ahringer},
  journal={Nature},
  year={2003},
  volume={421},
  pages={231-237}
}
A principal challenge currently facing biologists is how to connect the complete DNA sequence of an organism to its development and behaviour. Large-scale targeted-deletions have been successful in defining gene functions in the single-celled yeast Saccharomyces cerevisiae, but comparable analyses have yet to be performed in an animal. Here we describe the use of RNA interference to inhibit the function of ∼86% of the 19,427 predicted genes of C. elegans. We identified mutant phenotypes for 1… Expand
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