Systematic exploration of thioredoxin target proteins in plant mitochondria.

  title={Systematic exploration of thioredoxin target proteins in plant mitochondria.},
  author={Keisuke Yoshida and Ko Noguchi and Ken Motohashi and Toru Hisabori},
  journal={Plant & cell physiology},
  volume={54 6},
The thioredoxin (Trx) system is known to play a pivotal role in cellular redox regulation, but its target proteins in plant mitochondria remain largely uncharacterized. In this study, we systemically screened Trx target candidates in plant mitochondria. Mitochondrial protein extracts were prepared from Arabidopsis shoots, spinach leaves and potato tubers, and then subfractionated into soluble matrix and insoluble membrane fractions. Protein extracts were loaded onto an affinity column… CONTINUE READING

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