Systematic exploration of essential yeast gene function with temperature-sensitive mutants

  title={Systematic exploration of essential yeast gene function with temperature-sensitive mutants},
  author={Zhijian Li and Franco J. Vizeacoumar and Sondra Bahr and Jingjing Li and Jonas Warringer and Frederick S. Vizeacoumar and Renqiang Min and Benjamin VanderSluis and Jeremy Bellay and Michael Devit and James A. Fleming and Andrew Stephens and Julian Haase and Zhen-Yuan Lin and Anastasia Baryshnikova and Hong Lu and Zhun Yan and Ke Jin and Sarah A. Barker and Alessandro Datti and Guri Giaever and Corey Nislow and Chris Bulawa and Chad L. Myers and Michael Costanzo and Anne-Claude Gingras and Zhaolei Zhang and Anders Blomberg and Kerry Bloom and Brenda Andrews and Charles Boone},
  journal={Nature Biotechnology},
Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45%) of the 1,101 essential yeast genes, with ∼30% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to a kanMX selectable marker, allowing further genetic manipulation by synthetic genetic array (SGA… CONTINUE READING
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