Systematic exploration of error sources in pyrosequencing flowgram data

  title={Systematic exploration of error sources in pyrosequencing flowgram data},
  author={Susanne Balzer and Ketil Malde and Inge Jonassen},
  booktitle={Bioinformatics [ISMB/ECCB]},
MOTIVATION 454 pyrosequencing, by Roche Diagnostics, has emerged as an alternative to Sanger sequencing when it comes to read lengths, performance and cost, but shows higher per-base error rates. Although there are several tools available for noise removal, targeting different application fields, data interpretation would benefit from a better understanding of the different error types. RESULTS By exploring 454 raw data, we quantify to what extent different factors account for sequencing… CONTINUE READING
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The effect of sequencing errors on metagenomic gene prediction

  • S. M. Huse
  • BMC Genomics
  • 2009

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