Systematic evaluation of bias in microbial community profiles induced by whole genome amplification.

@article{Direito2014SystematicEO,
  title={Systematic evaluation of bias in microbial community profiles induced by whole genome amplification.},
  author={Susana O L Direito and Egija Zaura and Miranda Little and Pascale Ehrenfreund and Wilfred F. M. R{\"o}ling},
  journal={Environmental microbiology},
  year={2014},
  volume={16 3},
  pages={643-57}
}
Whole genome amplification methods facilitate the detection and characterization of microbial communities in low biomass environments. We examined the extent to which the actual community structure is reliably revealed and factors contributing to bias. One widely used [multiple displacement amplification (MDA)] and one new primer-free method [primase-based whole genome amplification (pWGA)] were compared using a polymerase chain reaction (PCR)-based method as control. Pyrosequencing of an… CONTINUE READING
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