Systematic design methodology for robust genetic transistors based on I/O specifications via promoter-RBS libraries

@inproceedings{Lee2013SystematicDM,
  title={Systematic design methodology for robust genetic transistors based on I/O specifications via promoter-RBS libraries},
  author={Yi-Ying Lee and Chih-Yuan Hsu and Ling-Jiun Lin and Chih-Chun Chang and Hsiao-Chun Cheng and Tsung-Hsien Yeh and Rei-Hsing Hu and Che Lin and Zhen Xie and Bor-Sen Chen},
  booktitle={BMC Systems Biology},
  year={2013}
}
Synthetic genetic transistors are vital for signal amplification and switching in genetic circuits. However, it is still problematic to efficiently select the adequate promoters, Ribosome Binding Sides (RBSs) and inducer concentrations to construct a genetic transistor with the desired linear amplification or switching in the Input/Output (I/O) characteristics for practical applications. Three kinds of promoter-RBS libraries, i.e., a constitutive promoter-RBS library, a repressor-regulated… CONTINUE READING