Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

Abstract

Cloning of multiple genes in a single vector has greatly facilitated both basic and translational studies that require co-expression of multiple factors or multi-units of complex protein. Many strategies have been adopted, among which 2A “self-cleaving” peptides have garnered increased interest for their polycistronic nature, small size and high “cleavage… (More)
DOI: 10.1038/s41598-017-02460-2

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