Systematic circular permutation of an entire protein reveals essential folding elements

  title={Systematic circular permutation of an entire protein reveals essential folding elements},
  author={Masahiro Iwakura and Tsutomu Nakamura and Chiori Yamane and Kosuke Maki},
  journal={Nature Structural Biology},
The importance of chain connectivity in determining protein function and stability can be examined by breaking the peptide backbone using a technique such as circular permutation. Cleavage at certain positions results in a complete loss of the ability of the protein to fold. When such cleavage sites occur sequentially in the primary structure, we call the region a ‘folding element’, a new concept that could assist in our understanding of the protein folding problem. The folding elements of… CONTINUE READING

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