Systematic chromosomal deletion of bacterial ribosomal protein genes.

  title={Systematic chromosomal deletion of bacterial ribosomal protein genes.},
  author={Shinichiro Shoji and Corey M. Dambacher and Zahra Shajani and James R. Williamson and Peter G. Schultz},
  journal={Journal of molecular biology},
  volume={413 4},
Detailed studies of ribosomal proteins (RPs), essential components of the protein biosynthetic machinery, have been hampered by the lack of readily accessible chromosomal deletions of the corresponding genes. Here, we report the systematic genomic deletion of 41 individual RP genes in Escherichia coli, which are not included in the Keio collection. Chromosomal copies of these genes were replaced by an antibiotic resistance gene in the presence of an inducible, easy-to-exchange plasmid-born… CONTINUE READING
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