Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.

@article{Brooks2010SystematicBA,
  title={Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.},
  author={Mark Adrian Brooks and Kamil Gewartowski and Eirini Mitsiki and Juliette L{\'e}toquart and Roland A. Pache and Ysaline Billier and Michela Bertero and Margot Correa and Mariusz Czarnocki-Cieciura and Michał Dadlez and V{\'e}ronique Henriot and Noureddine Lazar and Lila Delbos and Doroth{\'e}e Lebert and Jan Piwowarski and Pascal Rochaix and B B{\"o}ttcher and Luis Serrano and Bertrand S{\'e}raphin and Herman van Tilbeurgh and Patrick Aloy and Anastassis Perrakis and Andrzej Dziembowski},
  journal={Structure},
  year={2010},
  volume={18 9},
  pages={1075-82}
}
For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection… CONTINUE READING