Systematic approach for validating the ubiquitinated proteome.

  title={Systematic approach for validating the ubiquitinated proteome.},
  author={Nicholas T Seyfried and Ping Xu and Duc M Duong and Dongmei Cheng and John J. Hanfelt and Junmin Peng},
  journal={Analytical chemistry},
  volume={80 11},
Protein ubiquitination plays an essential regulatory role within all eukaryotes. Large-scale analyses of ubiquitinated proteins are usually performed by combining affinity purification strategies with mass spectrometry. However, there is no reliable method to systematically differentiate ubiquitinated species from copurified unmodified components. Here we report a simple strategy for the large-scale validation of ubiquitination by reconstructing virtual Western blots for proteins analyzed by… CONTINUE READING