Systematic analysis of yeast strains with possible defects in lipid metabolism

  title={Systematic analysis of yeast strains with possible defects in lipid metabolism},
  author={Günther Daum and Gabriele Tuller and T. Němec and Cladia Hrastnik and Gianni Balliano and Luigi Cattel and Paola Milla and Flavio Rocco and Aadreas Conzelmann and Christine Vionnet and Diane E. Kelly and Steven L. Kelly and Eckhard Schweizer and Hans-Joachim Sch{\"u}ller and Ursula Hojad and Eva Greiner and Kerin Finger},
Lipids are essential components of all living cells because they are obligate components of biological membranes, and serve as energy reserves and second messengers. Many but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of the yeast Saccharomyces cerevisiae have been cloned and gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between… 
Lipid composition of subcellular membranes of an FY1679‐derived haploid yeast wild‐type strain grown on different carbon sources
The lipid composition of yeast subcellular membranes reflects in a significant way the nutrient conditions caused by variation of the carbon source.
Isolation and characterization of a mutant defective in utilization of exogenous phosphatidylethanolamine in Saccharomyces cerevisiae.
Information is obtained on the utilization of exogenous PE in yeast by isolated mutants defective in utilization ofExogenous PE to support growth when the synthesis of PE was suppressed, and analyzed one of them.
Insight into yeast: A study model of lipid metabolism and terpenoid biosynthesis
With the development of transcriptomics, metabolomics, proteomics, and mathematical modeling, yeast Saccharomyces cerevisiae is recently considered as a model studying strain by biologists who try to
Dynamics of neutral lipid storage in yeast.
Besides biochemical properties of enzymes involved in yeast neutral lipid synthesis and degradation, regulatory aspects of these pathways and cell biological consequences of neutral lipid depletion will be discussed in this minireview.
A Yeast Strain Lacking Lipid Particles Bears a Defect in Ergosterol Formation*
It is demonstrated that TAG synthesis contributes more efficiently to lipid particle proliferation than synthesis of STE, and proteins typically located to lipid particles in wild type such as Erg1p, Erg6p, and Ayr1p are refined to microsomal fractions of the dga1lro1are1are2 quadruple mutant.
Comparative Lipidomic Profiling of S. cerevisiae and Four Other Hemiascomycetous Yeasts
The comparative lipidomic profiling of Saccharomyces cerevisiae, SacCharomyces bayanus, Kluyveromyces thermotolerans, Pichia angusta, and Yarrowia lipolytica revealed characteristic GP profiles for each strain, however, genetically related yeast strains show similarities in their GP compositions.
Sphingolipid functions in Saccharomyces cerevisiae.
Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast
Overall, the relative content of lipid species with saturated FA increased in detriment of those with unsaturated fatty acids, and the expression of the OLE1 mRNA was deregulated, but phospholipid fluidity changes were not observed.
Analyzing and Understanding Lipids of Yeast: A Challenging Endeavor.
  • S. Kohlwein
  • Biology, Chemistry
    Cold Spring Harbor protocols
  • 2017
Yeast is a model organism that provides several advantages for studying lipid metabolism, because most biosynthetic pathways are well described and a great deal of information is available on the regulatory mechanisms that control lipid homeostasis.


Saccharomyces cerevisiae cho2 mutants are deficient in phospholipid methylation and cross-pathway regulation of inositol synthesis.
Interestingly, cho2 mutants and other mutant strains that produce reduced levels of methylated phospholipids are unable to properly repress synthesis of the cytoplasmic enzyme inositol-1-phosphate synthase.
Coordinate regulation of phospholipid biosynthesis in Saccharomyces cerevisiae: pleiotropically constitutive opi1 mutant
Results suggest that the gene product of the OPI1 locus participates in the coordinate regulation of phospholipid synthesis, and that in opi1 cells these activities are expressed constitutively.
Biosynthesis of mannosylinositolphosphoceramide in Saccharomyces cerevisiae is dependent on genes controlling the flow of secretory vesicles from the endoplasmic reticulum to the Golgi
It is demonstrated that the biosynthesis of MIPC, M(IP)2C, and a subclass if IPCs is dependent on genes that are required for the vesicular transport of proteins from the ER to the Golgi.
Characterization, quantification and subcellular localization of inositol-containing sphingolipids of the yeast, Saccharomyces cerevisiae.
The presence of inositol-containing sphingolipids in organelles of the protein-secretory pathway strongly supports the notion that protein secretion and intracellular trafficking of sphingoipids are linked processes.
The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant
It is very likely that the TGL2 gene can complement the E. coli diacylglycerol kinase disruptant, because it encodes a protein that degrades the diACYlglycersol accumulated after growth in the presence of arbutin.
Molecular cloning and physical analysis of an 8·2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes
The nucleotide sequence of 6472 base pairs of an 8·2 kb segment of Saccharomyces cerevisiae chromosome XI has been determined and the transcripts of these genes represent approximately 83% of the DNA fragment, making it one of the most highly transcribed regions of the yeast chromosome analysed to date.
New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
A dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA is constructed and tested, and some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10–3–10–4.