Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies.

@article{Speicher2000SystematicAO,
  title={Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies.},
  author={Kaye D. Speicher and O Kolbas and Steven R. Harper and David W. Speicher},
  journal={Journal of biomolecular techniques : JBT},
  year={2000},
  volume={11 2},
  pages={74-86}
}
Metabolically radiolabeled recombinant proteins were used to systematically evaluate peptide recoveries from in-gel trypsin digestion.At least 80% of the labeled tryptic peptides could be readily extracted from gel bands containing 1 to 10 pmol, and at least 70% could be extracted at 200- to 500-fmol levels using a recombinant 52-kd protein. Alkylation before electrophoresis or before trypsin digestion had minimal effects on peptide recovery; although alkylation, especially before gel analysis… CONTINUE READING
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