Systematic analysis of essential genes reveals important regulators of G protein signaling.

@article{Cappell2010SystematicAO,
  title={Systematic analysis of essential genes reveals important regulators of G protein signaling.},
  author={Steven D. Cappell and Rachael A Baker and Dorota Skowyra and Henrik G. Dohlman},
  journal={Molecular cell},
  year={2010},
  volume={38 5},
  pages={
          746-57
        }
}
The yeast pheromone pathway consists of a canonical heterotrimeric G protein and MAP kinase cascade. To identify additional signaling components, we systematically evaluated 870 essential genes using a library of repressible-promoter strains. Quantitative transcription-reporter and MAPK activity assays were used to identify strains that exhibit altered pheromone sensitivity. Of the 92 newly identified essential genes required for proper G protein signaling, those involved with protein… CONTINUE READING
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