System-wide identification of wild-type SUMO-2 conjugation sites

  title={System-wide identification of wild-type SUMO-2 conjugation sites},
  author={Ivo A Hendriks and R C D'souza and Jer-Gung Chang and Matthias Mann and Alfred C O Vertegaal},
  booktitle={Nature communications},
SUMOylation is a reversible post-translational modification (PTM) regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry (MS) has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here we present a SUMO-specific protease-based methodology which circumvents this problem, dubbed Protease-Reliant Identification of SUMO Modification (PRISM). PRISM allows for detection of SUMOylated proteins as well as identification of… CONTINUE READING

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