Syringe loading introduces macromolecules into living mammalian cell cytosol.

@article{Clarke1992SyringeLI,
  title={Syringe loading introduces macromolecules into living mammalian cell cytosol.},
  author={Mark S. F. Clarke and Paul McNeil},
  journal={Journal of cell science},
  year={1992},
  volume={102 ( Pt 3)},
  pages={533-41}
}
We describe a simple, efficient, gentle and inexpensive technique for the introduction of normally impermeant macromolecules into the cytosol of living mammalian cells growing in suspension or attached to the culturing substratum. Loading is achieved by the production of transient, survivable plasma membrane disruptions as cells are passed back and forth through a standard syringe needle or similar narrow orifice. The loading volume required, which contains cells and the macromolecule to be… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 42 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Nuclear reassembly

  • L. Muthukrishnan, E. Warder, P. L. McNeil
  • 1991
1 Excerpt

Nuclear reassembly fibroblast growth factor is efficiently released from a cytosolic excludes large macromolecules

  • L. Muthukrishnan, E. Warder, P. L. McNeil, J. A. Swanson
  • Science
  • 1987

substitute

  • G. Magnusson, T. Olsson, J. A. Nyberg
  • 1986

Similar Papers

Loading similar papers…