Synthetic spatially graded Rac activation drives cell polarization and movement.

@article{Lin2012SyntheticSG,
  title={Synthetic spatially graded Rac activation drives cell polarization and movement.},
  author={Benjamin Lin and Chiaochun Joanne Wang and Tasuku Ueno and Andrew Harwell and Leah Edelstein-Keshet and Takanari Inoue and Andre Levchenko},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 52},
  pages={
          E3668-77
        }
}
Migrating cells possess intracellular gradients of active Rho GTPases, which serve as central hubs in transducing signals from extracellular receptors to cytoskeletal and adhesive machinery. However, it is unknown whether shallow exogenously induced intracellular gradients of Rho GTPases are sufficient to drive cell polarity and motility. Here, we use microfluidic control to generate gradients of a small molecule and thereby directly induce linear gradients of active, endogenous Rac without… CONTINUE READING
Highly Cited
This paper has 38 citations. REVIEW CITATIONS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

Spatial Manipulation with Microfluidics

Front. Bioeng. Biotechnol. • 2015
View 4 Excerpts
Highly Influenced

Computational modeling of single‐cell mechanics and cytoskeletal mechanobiology

Wiley interdisciplinary reviews. Systems biology and medicine • 2018
View 1 Excerpt

Stability analysis in spatial modeling of cell signaling.

Wiley interdisciplinary reviews. Systems biology and medicine • 2018
View 1 Excerpt