Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling.

@article{Brockmann2011SyntheticSA,
  title={Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling.},
  author={Eeva-Christine Brockmann and Sultana Akter and Tanja I Savukoski and Tuomas Huovinen and Ari Lehmusvuori and Janne Leivo and O Saavalainen and A. V. Azhayev and Timo L{\"o}vgren and Jukka Hellman and Urpo J Lamminm{\"a}ki},
  journal={Protein engineering, design & selection : PEDS},
  year={2011},
  volume={24 9},
  pages={691-700}
}
Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv antibody library was constructed in single immunoglobulin framework to enable rapid affinity maturation by updated Kunkel's mutagenesis. The initial diversity was generated predominantly in the V(H) domain combined with only 36 V(L) domain variants yielding 3 × 10(10) unique members in the phage-displayed library. After three rounds of panning… CONTINUE READING