Synthetic sandwich culture of 3D hepatocyte monolayer.

  title={Synthetic sandwich culture of 3D hepatocyte monolayer.},
  author={Yanan Du and Rongbin Han and Feng Wen and Susanne Ng San San and Lei Xia and Thorsten Wohland and Hwa Liang Leo and Hanry Yu},
  volume={29 3},
The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions. Applications of the ECM-based sandwich culture are limited by the mass transfer barriers induced by the top gelled ECM layer, complex molecular composition of ECM with batch-to-batch variation and uncontrollable coating of the ECM double layers. We have addressed these limitations of the ECM… CONTINUE READING


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Sorption of low-molecular-weight anions into thin polycation layers grafted onto a film. Langmuir 1993;9:1121–4

  • EYU Uchida, Y. Ikada
  • 1993
Highly Influential
2 Excerpts

Characterization of poly(acrylic acid) diffusion dynamics on the grafted surface of poly(ethylene terephthalate) films by fluorescence correlation spectroscopy

  • X Pan, C Aw, Y Du, H Yu, T. Wohland
  • Biophys Rev Lett 2006;1(4):433
  • 2006
1 Excerpt

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