Synthetic protein scaffolds provide modular control over metabolic flux

@article{Dueber2009SyntheticPS,
  title={Synthetic protein scaffolds provide modular control over metabolic flux},
  author={John E. Dueber and Gabriel C. Wu and G Reza Malmirchegini and Tae Seok Moon and Christopher J. Petzold and Adeeti V. Ullal and Kristala Jones Prather and Jay D. Keasling},
  journal={Nature Biotechnology},
  year={2009},
  volume={27},
  pages={753-759}
}
Engineered metabolic pathways constructed from enzymes heterologous to the production host often suffer from flux imbalances, as they typically lack the regulatory mechanisms characteristic of natural metabolism. [] Key Result The natural modularity of these domains enabled us to optimize the stoichiometry of three mevalonate biosynthetic enzymes recruited to a synthetic complex and thereby achieve 77-fold improvement in product titer with low enzyme expression and reduced metabolic load.

Spatial organization of enzymes for metabolic engineering.

Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux

A pair of short peptide tags (RIAD and RIDD) are chosen to create scaffold-free enzyme assemblies and synthetic metabolic nodes to impose metabolic control in biosynthetic microbe factories.

Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.

The importance of protein engineering for advancing metabolic engineering of secondary metabolism pathways through integrated enzyme optimization can enhance the collective toolkit of microbial engineering to shape the future of chemical manufacturing.

[Research progress on synthetic scaffold in metabolic engineering - a review].

The application of synthetic scaffolds in metabolic engineering is summarized, the main principle of scaffold designs are outlined, and the current challenges in their application are highlighted.

Protein design for pathway engineering.

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

This work reports a simple, efficient and programmable approach named ‘customized optimization of metabolic pathways by combinatorial transcriptional engineering (COMPACTER)’ for rapid tuning of gene expression in a heterologous pathway under distinct metabolic backgrounds.

Constructing de novo biosynthetic pathways for chemical synthesis inside living cells.

Efforts to understand the relationship among sequence, structure, and function in the basic biochemical sciences can advance these goals for synthetic biology applications while also serving as an experimental platform for elucidating the in vivo specificity and function of enzymes and reconstituting complex biochemical traits for study in a living model organism.

Light-based control of metabolic flux through assembly of synthetic organelles

It is reported that optogenetic control can be used to extend compartmentalization and dynamic control to engineered metabolisms in yeast and enhances product formation and specificity during deoxyviolacein biosynthesis by decreasing concentrations of intermediate metabolites and reducing flux through competing pathways.

Synthetic scaffolds for pathway enhancement.

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