Synthetic protein scaffolds provide modular control over metabolic flux

@article{Dueber2009SyntheticPS,
  title={Synthetic protein scaffolds provide modular control over metabolic flux},
  author={J. Dueber and G. Wu and G. R. Malmirchegini and T. Moon and C. Petzold and Adeeti V. Ullal and K. Prather and J. Keasling},
  journal={Nature Biotechnology},
  year={2009},
  volume={27},
  pages={753-759}
}
Engineered metabolic pathways constructed from enzymes heterologous to the production host often suffer from flux imbalances, as they typically lack the regulatory mechanisms characteristic of natural metabolism. [...] Key Result The natural modularity of these domains enabled us to optimize the stoichiometry of three mevalonate biosynthetic enzymes recruited to a synthetic complex and thereby achieve 77-fold improvement in product titer with low enzyme expression and reduced metabolic load.Expand
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