Synthetic peptides as antigens: pitfalls of conjugation methods.

  title={Synthetic peptides as antigens: pitfalls of conjugation methods.},
  author={Jean Paul Briand and Sylviane Muller and Marc H. V. Van Regenmortel},
  journal={Journal of immunological methods},
  volume={78 1},
Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine) were found to elicit specific antibodies that reacted with unrelated carrier proteins treated with the same coupling agent. To demonstrate the presence of peptide antibodies in an antiserum raised against a peptide-carrier… CONTINUE READING

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