Synthetic modular systems – reverse engineering of signal transduction

@article{Pawson2005SyntheticMS,
  title={Synthetic modular systems – reverse engineering of signal transduction},
  author={Tony Pawson and Rune Linding},
  journal={FEBS Letters},
  year={2005},
  volume={579}
}

Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring.

This work combines high-throughput proteomics with the MMG probabilistic tool, which integrates the data with the metabolic circuit's topology to study the global metabolic effects of the insertion of synthetic circuits in a cellular chassis.

The promise of synthetic biology

  • J. Pleiss
  • Biology, Engineering
    Applied Microbiology and Biotechnology
  • 2006
The design concept and examples from four fields of application (genetic circuits, protein design, platform technologies, and pathway engineering) are discussed, which demonstrate the usefulness and the promises of synthetic biology.

Designing new cellular signaling pathways.

Systems and Synthetic Biology in E. coli Cells Quantitative System Characterization, Programming and Engineering Novel Cellular Functions

The work showed that the level and variability of gene expression varied across different cell strains, and it suggested that gene circuit modules from a standard library cannot be used universally; the cellular context and the time dependent dynamics must be considered when implementing gene circuits.

Peptide and protein building blocks for synthetic biology: from programming biomolecules to self-organized biomolecular systems.

This work describes how, for certain protein-folding motifs, polypeptide chains can be instructed to fold and combined to give structured complexes, and how protein-based systems may be encapsulated to control and investigate their functions.

Overview of Signal Transduction

An overview of common features of cellular signaling pathways, including their interactions and responses to environmental stimuli, focuses on the regulation of signaling pathways by protein functional‐domain interactions as well as the intracellular proteins that mediate signal transduction.

Designing biological systems.

The scientific accomplishments in synthetic biology are described, as well as its forays into biological part standardization and education of future biological designers.

Reconstruction of genetic circuits

Recent progress in this area of synthetic biology is described, highlighting newly developed genetic components and biological lessons learned from this approach.

Rule-based modeling of biochemical systems with BioNetGen.

This work focuses on how a rule-based model is specified in the BioNetGen language (BNGL) and how a model specification is analyzed using the Bio netGen software tool.

Rules for Modeling Signal-Transduction Systems

Approaches to creation of mathematical models of signaling systems with strategies that keep the models from being unwieldy but still allow them to accurately reflect biological systems are reviewed.

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