Synthetic lethal screen.

@article{Barbour2006SyntheticLS,
  title={Synthetic lethal screen.},
  author={Leslie Barbour and Wei Xiao},
  journal={Methods in molecular biology},
  year={2006},
  volume={313},
  pages={161-9}
}
The synthetic lethal screen is a method of isolating novel mutants whose survival is dependent on a gene of interest. Combining the colony-color assay with a synthetic lethal screen offers a means to visually detect a mutant that depends on a plasmid for survival. Screening for synthetic lethals can be achieved in four steps. First, the gene of interest… CONTINUE READING