Synthetic guinea pig insulin B-chain C-terminal decapeptide: a novel immunogen for generating immunocytochemical-grade antisera.

@article{Maimon1991SyntheticGP,
  title={Synthetic guinea pig insulin B-chain C-terminal decapeptide: a novel immunogen for generating immunocytochemical-grade antisera.},
  author={J J Maimon and Marina Mauro and K. C. Gorray and Bradley S Schneider},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1991},
  volume={39 11},
  pages={1571-4}
}
Antisera to guinea pig insulin are not commonly available, largely because of the short supply and limited immunogenicity of the intact hormone. To overcome these problems we have employed a novel reagent, synthetic guinea pig insulin B-chain C-terminal decapeptide, as a hapten for raising antibodies that react with intact guinea pig insulin. The decapeptide, coupled to bovine serum albumin, was successfully used as an immunogen in rabbits. The resulting anti-serum was employed for… CONTINUE READING

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