Synthetic genes for human muscle-type adenylate kinase in Escherichia coli.

@article{Kim1989SyntheticGF,
  title={Synthetic genes for human muscle-type adenylate kinase in Escherichia coli.},
  author={H. Kim and S. Nishikawa and T. Tanaka and S. Uesugi and H. Takenaka and M. Hamada and S. Kuby},
  journal={Protein engineering},
  year={1989},
  volume={2 5},
  pages={
          379-86
        }
}
  • H. Kim, S. Nishikawa, +4 authors S. Kuby
  • Published 1989
  • Biology, Medicine
  • Protein engineering
  • An artificial gene coding for the human muscle-type cytosolic adenylate kinase (hAK1) was chemically synthesized and directly expressed in Escherichia coli under the control of trp promoter. The DNA duplex of 596 bp was designed and constructed from 40 oligonucleotide fragments of typically 30 nucleotides in length. Twelve unique restriction sites were fairly evenly spaced in the synthetic gene to facilitate site-specific mutagenesis at any part of this recombinant protein. The genes for mutant… CONTINUE READING
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