Synthetic fragments of beta-casein as model substrates for liver and mammary gland casein kinases.

@article{Meggio1989SyntheticFO,
  title={Synthetic fragments of beta-casein as model substrates for liver and mammary gland casein kinases.},
  author={Flavio Meggio and John W. Perich and Helmut E. Meyer and Edeltraut Hoffmann-Posorske and Donald P. Lennon and Richard B. Johns and Lorenzo A Pinna},
  journal={European journal of biochemistry},
  year={1989},
  volume={186 3},
  pages={
          459-64
        }
}
The octapeptide Glu-Ser-Leu-Ser-Ser-Ser-Glu-Glu, corresponding to the 14-21 sequence of bovine beta-casein A2 and 11 shorter and/or modified derivatives were synthesized and used as model substrates for three casein kinases: rat liver casein kinases 2 and 1 and a casein kinase isolated from the golgi-enriched fraction of lactating mammary gland (GEF-casein kinase). Casein kinase-2 readily phosphorylates the octapeptide at its Ser-4 residue with a Vmax value comparable to those obtained with… CONTINUE READING

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Novel consensus sequence for the Golgi apparatus casein kinase, revealed using proline-rich protein-1 (PRP1)-derived peptide substrates.

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