Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR-Cas9-Mediated Genome Editing in Yarrowia lipolytica.

@article{Schwartz2016SyntheticRP,
  title={Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR-Cas9-Mediated Genome Editing in Yarrowia lipolytica.},
  author={Cory M. Schwartz and Murtaza Shabbir Hussain and Mark A Blenner and Ian Wheeldon},
  journal={ACS synthetic biology},
  year={2016},
  volume={5 4},
  pages={356-9}
}
The oleaginous yeast Yarrowia lipolytica is a valuable microbial host for chemical production because it has a high capacity to synthesize, modify, and store intracellular lipids; however, rapid strain development has been hampered by the limited availability of genome engineering tools. We address this limitation by adapting the CRISPR-Cas9 system from Streptococcus pyogenes for markerless gene disruption and integration in Y. lipolytica. Single gene disruption efficiencies of 92% and higher… CONTINUE READING
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