Synthetic DNA delivery systems

  title={Synthetic DNA delivery systems},
  author={Dan Luo and W. Mark Saltzman},
  journal={Nature Biotechnology},
The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene… 

Challenges in DNA Delivery and Recent Advances in Multifunctional Polymeric DNA Delivery Systems.

A comprehensive overview of the interactions between the protein corona and DNA vector, the mechanisms and challenges of nonviral DNA vectors, and important concepts in the design of DNA carriers identified via past reports on DNA delivery systems are provided.

Nanotechnology and DNA Delivery

This review article will focus on the recent developments in nanoscale DNA delivery systems that consist of chemical dendrimers, DNA dendromers, nanospheres, nanolayers, nanorods, and nanotubes.

Cellular Fate of a Modular DNA Delivery System Mediated by Silica Nanoparticles

It is shown that the modular system based upon concentration of DNA at the level of the cell can be used to increase transfection efficiency, and that further modification of the system may better target DNA delivery and overcome other barriers of DNA expression.

Efficient active transport of gene nanocarriers to the cell nucleus

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  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
The results provide direct evidence to dispute the common belief that the efficiency of nonviral gene carriers is dramatically reduced because of the need for their relatively slow random diffusion through the cell cytoplasm to the nucleus and, instead, focuses the attention of rational carrier design on overcoming barriers downstream of perinuclear accumulation.

Gene therapy and DNA delivery systems.

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This chapter describes a new peptide-based gene delivery system, MPG, which forms stable noncovalent complexes with oligonucleotides and promotes their delivery into a large panel of cell lines without the need for prior chemical covalent coupling.

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The aim of this work was to develop and optimize a tool that could offer simultaneous quantitative information both on the intracellular dissociation of oligonucleotides from lipid nanoparticles, and on the DNA escape from endocytic compartments.

Nucleocytoplasmic transport of plasmid DNA: a perilous journey from the cytoplasm to the nucleus.

This paper reviews the major metabolic and physical impediments that plasmid DNA vectorized by synthetic vectors encounters between the cytosol and the nucleus and reviews some of the current methods of gene transfer mediated by synthetic vector, highlighting systems that exploit the actual knowledge of the nucleocytoplasmic transport of plasmids.

Quantitative evaluation of DNA dissociation from liposome carriers and DNA escape from endosomes during lipid-mediated gene delivery.

The aim of this work was to develop and optimize a tool that could offer simultaneous quantitative information both on the intracellular dissociation of oligonucleotides from lipid nanoparticles, and on the DNA escape from endocytic compartments, and to follow in real time both of these processes simultaneously.



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This review will provide a critical discussion of the merits and limitations of liposomal and polycationic carrier systems for gene transfer from the viewpoints of their physicochemical and pharmacokinetic properties.

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Three types of lipidic gene transfer vectors are described and DNA encapsulated in neutral or anionic liposomes, and liposome-entrapped, polycation-condensed DNA (LPDI and LPDII) are described.

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It is demonstrated that vector unpackaging should be added to the list of barriers to receptor-mediated polyplex gene delivery, thus providing an additional design principle for targeted synthetic delivery vehicles.

Liver‐directed gene transfer: a linear polyethylenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo

Efficient DNA delivery is a prerequisite for the successful implementation of molecular antiviral strategies against chronic viral hepatitis and gene therapy in general. The cationic polymer

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This review summarizes lipid-based technologies for the delivery of nucleic acid-based drugs and introduces a new class of carrier systems, which solve the conflicting demands of circulation longevity and intracellular delivery.

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  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
It is found that complexes attach to cell surfaces and migrate into clumps that are endocytosed PEI, whether administered with or without DNA, undergoes nuclear localization in the form of ordered structures.

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.