Synthetic CpG islands reveal DNA sequence determinants of chromatin structure

@inproceedings{Wachter2014SyntheticCI,
  title={Synthetic CpG islands reveal DNA sequence determinants of chromatin structure},
  author={Elisabeth Wachter and Timo Quante and Cara Merusi and Aleksandra Arczewska and Francis A Stewart and Shaun Webb and Adrian Bird and Anne C Ferguson-Smith},
  booktitle={eLife},
  year={2014}
}
The mammalian genome is punctuated by CpG islands (CGIs), which differ sharply from the bulk genome by being rich in G + C and the dinucleotide CpG. CGIs often include transcription initiation sites and display 'active' histone marks, notably histone H3 lysine 4 methylation. In embryonic stem cells (ESCs) some CGIs adopt a 'bivalent' chromatin state bearing simultaneous 'active' and 'inactive' chromatin marks. To determine whether CGI chromatin is developmentally programmed at specific genes or… CONTINUE READING