Synthesis of wheat leaf nitrite reductase de novo following induction with nitrate and light.

@article{Small1984SynthesisOW,
  title={Synthesis of wheat leaf nitrite reductase de novo following induction with nitrate and light.},
  author={Ian S. Small and J. C. Gray},
  journal={European journal of biochemistry},
  year={1984},
  volume={145 2},
  pages={291-7}
}
Nitrite reductase has been purified almost 3000-fold, in 35% yield, to a specific activity of 77 units (mg protein)-1 from wheat leaves using a multi-step procedure with affinity chromatography on ferredoxin-Sepharose as the final step. The purified enzyme, although not homogeneous, exhibited absorption maxima at 278, 390, 568 and 687 nm. Minor contaminants… CONTINUE READING